Showing posts with label biotechnology. Show all posts
Showing posts with label biotechnology. Show all posts

Wednesday, August 03, 2011

Genetically engineered algae for biofuel?

Scientists in the US are developing genetically engineered strains of algae, mostly cyanobacteria ("blue green algae") to produce alkane fuels in quantity. They are confident that algal biofuel avoids many of the drawbacks of corn- or grass-biofuels, such as competition with food supply, low yields per hectare, and need for extensive processing.

However, they're running into problems of their own: need for large volumes of water, difficult in scaling up the process from the lab to the field, and the high volume of carbon dioxide consumption that these algae have. Some of the problems were unexpected, however:
"Shrimp think algae are good food," says CEO Pyle [of the company Sapphire Energy]. "If you don't pay attention, you will ultimately have a shrimp farm."
The returns on such an investment might not be too bad either!

Thursday, July 08, 2010

Norman Borlaug

Norman Borlaug, "The Father of the Green Revolution" passed away at the age of 95 on Sept 12 2009. He was instrumental in developing and introducing semi-dwarf, high-yield, disease resistant wheat varieties. NY times wrote of him as "...the plant scientist who did more than anyone else in the 20th Century to teach the world to feed itself..."

He won the Nobel Peace Prize for agricultural innovation and the development of high-yield crops in 1970. The Green Revolution has been touted to have averted a world-wide famine in the late 20th century.

Excerpts of his speech during the Nobel Prize award ceremony showed how committed he was in using science for the betterment of mankind:

"Accordingly, I shall not dwell upon the personal honor, for I have not done so even within myself. Instead, I want to devote my remarks to commendation of the Nobel Committee which had the perspicacity and wisdom to recognize the actual and potential contributions of agricultural production to prosperity and peace among the nations and peoples of the world.

Obviously, I am personally honored beyond all dreams by my election. But the obligations imposed by the honor are far greater than the honor itself, both as concerns me personally and also the army of hunger fighters in which I voluntarily enlisted a quarter of a century ago for a lifetime term. I am acutely conscious of the fact that I am but one member of that vast army and so I want to share not only the present honor but also the future obligations with all my companions in arms, for the Green Revolution has not yet been won.

It is true that the tide of the battle against hunger has changed for the better during the past three years. But tides have a way of flowing and then ebbing again. We may be at high tide now, but ebb tide could soon set in if we become complacent and relax our efforts. For we are dealing with two opposing forces, the scientific power of food production and the biologic power of human reproduction.

Man has made amazing progress recently in his potential mastery of these two contending powers. Science, invention, and technology have given him materials and methods for increasing his food supplies substantially and sometimes spectacularly, as I hope to prove tomorrow in my first address as a newly decorated and dedicated Nobel Laureate. Man also has acquired the means to reduce the rate of human reproduction effectively and humanely. He is using his powers for increasing the rate and amount of food production. But he is not yet using adequately his potential for decreasing the rate of human reproduction. The result is that the rate of population increase exceeds the rate of increase in food production in some areas.

There can be no permanent progress in the battle against hunger until the agencies that fight for increased food production and those that fight for population control unite in a common effort. Fighting alone, they may win temporary skirmishes, but united they can win a decisive and lasting victory to provide food and other amenities of a progressive civilization for the benefit of all mankind.

Then, indeed, Alfred Nobel's efforts to promote Brotherhood between nations and their peoples will become a reality.

Let our wills say that it shall be so."

Further reading:

Saturday, May 22, 2010

The first self-replicating artificial cell created

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We report the design, synthesis and assembly of the 1.08- Mbp Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a Mycoplasma capricolum recipient cell to create new Mycoplasma mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.


One year after synthesizing a synthetic genome, the Craig Venter Center finally published their results in Science. For this paper, Science has kindly allowed free access.

Of course this created alot of discussion amongst the scientific circle. Even President Obama penned a letter to the research team.

In the basic criteria of what life is like, most scientists agree that three basic components has to exist: a container, a way to harvest energy and an information carrier like RNA or another nucleic acid.

This is a step from similar protocellular work by Szostak from Havard Medical School. The Craig Venter Center team's bacteria can grow and divide.

Reading list suggested by Carl Zimmer.

 

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Sunday, January 27, 2008

Human Genome Project

I am relooking at some notes and slides I prepared for this topic. Its an interesting topic not only because of the science behind it but also the ethical, social and legal implications (that makes the acronym "ELSI") which blur the lines between right and wrong. Here are two very good resources

The first is the NOVA programs. The videos capture authentic situations that individuals face with regards to genetic diseases and also feature the scientists, science behind the project. Its more information packed in multimedia than I can summarize in a set of lecture notes. More information meaning the drama of the video that will contribute to affective learning and hence motivate students to learn more about the subject.

NOVA Online | Cracking the Code of Life | Watch the Program Here

The 2nd is this free,... yes free, online book you can download. Its easy to read and the examples giving illustrate clearly the perplexing "ELSI" issues about genetic testing, abortions, genetic determinism... that will surface once genetic tests become more accessible..

Your Genes, Your Choices: Exploring the Issues Raised by Genetic Research

Think ELSI and genetic testing are still far off... well read this groundbreaking and probably as "Brave New World" as it can get article in the New York Times on how you can pay just under USD$1000 for the company 23andMe to scan your genome for 580,000 SNPs (Single Nucleotide Polymorphisms). You actually can order the kit.. not sure how it works but I guess you can do it at home for yourself and children. There are at least 3 companies who will do that for around that price.
23andMe - Store - Build Your Order

Just spit..

23andMe - Store - Build Your Order


This NYT journalist got her own genome scanned by just spitting saliva into a little test tube . Here's a snippet of the article titled "My Genome, Myself: Seeking Clues in DNA"

"I don't like brussels sprouts. Who knew it was genetic? But I have the snippet of DNA that gives me the ability to taste a compound that makes many vegetables taste bitter. I differ from people who are blind to bitter taste -- who actually like brussels sprouts -- by a single spelling change in our four-letter genetic alphabet: somewhere on human chromosome 7, I have a G where they have a C."



But that's just information.... trivia about your genes. It gets more serious when people actually use that information to determine the genetics of their progeny. This couple actually had their daughter "genetically determined" so that she wouldn't inherit forms of genes that would make her highly susceptible to a certain form of colon cancer. This testing is termed preimplantation genetic diagnosis, or P.G.D., which means you need to cull embryos to get the right one. An eight-cell embryo has gone through fertilization.

Couples Cull Embryos to Halt Heritage of Cancer - New York Times

For this couple, 4 were culled leaving 10 good ones that didn't have those forms of genes that cause cancer, 2 more were culled when a Down's syndrome test was done. Think that is unethical? Well there are people who actually remove their large intestines or breasts as a prophylactic measure against such cancers once they find they harbour just the forms of genes that make them susceptible. They may have siblings who have died because of that disease. Watch the 3rd video called One Wrong Letter in the set of videos on Nova and you will know why it becomes so grey.

(thanks to Kevin Lam who pointed out the genome scan link).

Wednesday, November 21, 2007

Bacterial Biofilms

This recent article in PLoS Biology gives a very readable review of bacterial biofilms and the kinds of problems they pose in medical settings. A familiar example of a bacterial biofilm is plaque on our teeth. Bacteria, when they reach a certain critical density on a surface, begin to aggregate and secrete a slimy polymeric matrix which aids further establishment and eventually builds up to a complex structure with channels and pores through which fluid can flow, carrying in nutrients and carrying away waste materials.

A few interesting points to think about:

  • The means by which the bacteria detect the critical population density needed to form biofilms is called quorum sensing. How does it work?
  • Previously it was thought that the slimy matrix protected bacteria by preventing them from being engulfed or attacked by other cells or substances, but the matrix has to be permeable to most substances because waste and nutrients must reach the bacteria. So how do they defend themselves?
  • Biofilms usually form on surfaces over which there is regular fluid flow. Why would the biofilm growth form be advantageous?
  • Bacteria living in biofilms reproduce at a slower rate than free living ones. How might this be understood by analogy to life-history trait selection in r- and K-selective environments?

Friday, November 09, 2007

GM foods are ubiquitous

Almost all the varieties of rice grown in Southeast Asia are genetically modified. A conspiracy by governments and biotech firms to reap big bucks while penalising ignorant consumers and poor farmers? No. They have been 'modified' by "accidentally as the result of mutations, chromosomal recombinations, translocations of pieces of DNA and even deletions of sections of DNA. This rice is consumed everywhere without the requirement of any laboratory tests," as this article rightly points out. Are GM foods the enemy, as some organic food advocates say that they are, or is this moralising about GM foods costing lives which could be saved by improved crop yields?